|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
After 14 days of osteoblastic induction, osteoblast phenotypes were detected by ALP and calcium nodule staining, and the expression of BMP-2 and TGF-β1 was observed by western blotting.
|
31481070 |
2019 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
In addition, up-regulation of Fam50a also increased ALP activity and mineralized nodule formation in a dose-dependent manner.
|
28574578 |
2018 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
Subsequently, examination of mineralized nodule formation and evaluation of alkaline phosphatase (ALP) activity and ALP gene expression revealed that the most effective concentration of BDNF to elicit a response in hBMSCs was 100 ng/mL.
|
29490590 |
2018 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
In C-OPLL cells, leptin treatment led to a significant increase in mRNA expressions of ALP and OCN and formation of mineralized nodule.
|
29970120 |
2018 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
Sema4D significantly inhibited ALP activity and mineralized nodule formation of DPSCs.
|
28027822 |
2017 |
|
Nodule
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
Among the screened library, ethyl 4-(7-(sec-butyl)-2-(4-methylbenzoyl)benzofuran-5-yl)-2-methyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate (compound 21) significantly enhanced the ALP production and mineralized nodule formation, which are primary requisites in the process of in vitro osteogenesis.
|
29097030 |
2017 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
However, expression levels of Runx2, BSP, ephrinB2 and EphB4, as well as ALP activity and mineral nodule formation, were significantly enhanced in MC3T3-E1 cells treated with low concentrations of TNF-α.
|
27726217 |
2017 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
ALP activity and mineralized nodule formation test were used to evaluate bone formation.
|
28963864 |
2017 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
Furthermore, GILZ (dexamethasone-induced) inhibition caused by icariin or moderately silenced by GILZ siRNA abolished the osteogenesis inhibition effect of dexamethasone, as indicated by the changes in the GILZ, ALP, OPG and RANKL expression levels; ALP activity; and calcium nodule.
|
28363169 |
2017 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
Overexpression of PHF20 enhanced ALP activity and mineralized nodule formation as well as the expression of osteogenic markers including Runx2.
|
28808306 |
2017 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
The results indicated that ASA VI promoted the proliferation of OVX rBMSCs and enhanced ALP activity and calcified nodule formation.
|
27756897 |
2016 |
|
Nodule
|
0.100 |
AlteredExpression
|
phenotype |
BEFREE |
Transfection with shRNAs markedly increased mineralized nodule formation and the osteogenic-related markers ALP and OCN levels in hPDLCs, whereas the overexpression of S100A4 significantly reduced mineralized nodule formation, and increased the matrix degradation enzymes MMP-2 and MMP-13 levels in hPDLCs.
|
26499072 |
2015 |