Serum levels of miR-200c can been used to effectively distinguish oral squamous cell carcinoma patients from healthy control, and patients with lower serum level of miR-200c showed shorter survival time. miR-200c overexpression inactivated Akt and Glut1 expression, while Akt activator and Glut1 overexpression showed no significant effects on miR-200c.
We used quantitative real-time polymerase chain reaction (PCR) to compare relative expression of miR-21, miR-141, and miR-200c in 70 formalin-fixed, paraffin-embedded samples of sinonasal carcinoma tissue (majority of squamous cell carcinoma [SCC] samples) with 17 control samples of sinonasal tissue.
Aberrant miR-375 and miR-200a expression and miR-200c-141 methylation could be detected in and distinguish OSCC patient oral rinse and saliva from healthy volunteers, suggesting a potential clinical application for OSCC specific miRNA signatures in oral fluids.