In a combined in vivo-in vitro study where the lymphocytes had been isolated from a patient 2 hours after intake of 60-80 mg 8-MOP and then irradiated with therapeutic UVA doses, a significant increase in chromosomal aberrations was found.
In a combined in vivo-in vitro study where the lymphocytes had been isolated from a patient 2 hours after intake of 60-80 mg 8-MOP and then irradiated with therapeutic UVA doses, a significant increase in chromosomal aberrations was found.
In a combined in vivo-in vitro study where the lymphocytes had been isolated from a patient 2 hours after intake of 60-80 mg 8-MOP and then irradiated with therapeutic UVA doses, a significant increase in chromosomal aberrations was found.
The chromosome abnormalities were detected in B- as well as T-lymphocytes (as evidenced by using both PHA- and PWM-stimulated cultures) in all probands, but one was mosaic in PHA culture, although all his PWM-stimulated cells were abnormal.
This gene dosage effect was ascertained by (1) comparative measurements of the specific activity; (2) relating the specific activity of FH to that of reference enzymes, not influenced by the chromosomal anomaly; and (3) by immunoprecipitation methods, using a rabbit antiserum against pig heart FH which cross-reacts with the human enzyme.
By use of the galactose/NaB3H4 surface labeling technique followed by polyacrylamide slab gel electrophoresis, it is shown that the major labeled surface glycoprotein (GP130) of normal human blood granulocytes is markedly reduced in granulocytes from three patients with a chromosomal abnormality in all or most bone marrow mitoses.
By use of the galactose/NaB3H4 surface labeling technique followed by polyacrylamide slab gel electrophoresis, it is shown that the major labeled surface glycoprotein (GP130) of normal human blood granulocytes is markedly reduced in granulocytes from three patients with a chromosomal abnormality in all or most bone marrow mitoses.
By use of the galactose/NaB3H4 surface labeling technique followed by polyacrylamide slab gel electrophoresis, it is shown that the major labeled surface glycoprotein (GP130) of normal human blood granulocytes is markedly reduced in granulocytes from three patients with a chromosomal abnormality in all or most bone marrow mitoses.
Ataxia telangiectasia (A-T) is an inherited, recessive, cancer-prone disease with associated immunodeficiency and chromosome abnormalities involving TCR loci.
The frequent cytogenetic abnormality--isochromosome 17q [i(17)q]--observed in medulloblastomas (MB) may result in altered expression of the oncosuppressor gene p53 that is located on 17p. p53 expression was therefore evaluated in five MBs and in one MB cell line derived from one of these tumors.
Phenotypical characteristics of acute myelocytic leukemia associated with the t(8;21)(q22;q22)chromosomal abnormality: frequent expression of immature B-cell antigen CD19 together with stem cell antigen CD34.
In four of five rearranged cases the presence of a specific chromosomal abnormality involving chromosome 10 (cases 1 and 2) and chromosome 1 (cases 3 and 4) was associated with the rearrangement of two protooncogenes: RET and NTRKI (formerly trk), respectively, with different donor genes.
Although the well-described cytogenetic abnormalities associated with particular FAB subtypes in the West were observed, certain important local differences were noted.
A girl with ornithine transcarbamylase (OTC) deficiency was investigated for molecular and cytogenetic abnormalities that might explain this phenotype.
The establishment of an interleukin-6-dependent myeloma cell line (FLAM-76) carrying t(11;14)(q13;q32)chromosome abnormality from an aggressive nonsecretory plasma cell leukemia.
The induction of gene mutations and chromosome aberrations by plasmid pEJ6.6 carrying the activated c-Ha-ras-1 oncogene from human bladder carcinoma was studied in cultured Chinese hamster cells.