Using GFP-based reporters and Western blot analysis, we found that the representative human cystitis isolate <i>E. coli</i> UTI89 and its derivative UTI89ΔFimH, which does not bind to BECs, equally activate phosphatidylinositol 4,5-bisphosphate 3-OH kinase (PI3K), Akt kinase, and mTOR complex (mTORC) 1 and 2 in BECs.