The objectives of this study were: 1) to evaluate the method used for estimating the neuromuscular fatigue threshold from surface electromyographic amplitude (the PWCFT test) during a single incremental cycling workout using 1-minute exercise periods, and 2) to investigate the possible associations between PWCFT and metabolic (onset of blood lactate accumulation [OBLA]) and ventilatory (ventilatory threshold [VT] and respiratory compensation point [RCP]) variables.