This showed that the <i>K</i><sub>trans</sub> value might be a non-invasive method to evaluate the BTB permeability in rat glioma after microbubble-enhanced ultrasound treatment.Western blot, qRT-PCR and immunohistochemical staining revealed that MEUS increased the <i>K</i><sub>Ca</sub> channels expression and reduced JAM-A expression in glioma.