Methylation‑specific polymerase chain reaction analysis and bisulfite pyrosequencing confirmed hypermethylation of miR‑193b promoter regions in esophageal cancer and BE cells, whereas hypermethylation was not observed in normal esophageal squamous epithelial cells.
These results highlight the importance of miR-193b in determining oesophageal cancer cell viability and demonstrate an enhancement of chemotoxicity that is independent of apoptosis induction.