Profiling for active enhancers using the H3K27ac histone modification revealed UC-derived organoid enrichment for pathways indicative of gastrointestinal cancer, including S100 calcium-binding protein P (<i>S100P</i>), and revealed novel markers for GI cancer, including both <i>LYZ</i> and neuropeptide S receptor 1 (<i>NPSR1</i>).