In the present study, we assessed the prevalence of the <i>BRAF</i> V600E mutation in a large cohort of TSC related SEGAs (n=58 patients including 56 with clinical TSC) and found no evidence of either <i>BRAF</i> V600E or other mutations in <i>BRAF.</i> To confirm that these SEGAs fit the classic model of two hit <i>TSC1</i> or <i>TSC2</i> inactivation, we also performed massively parallel sequencing of these loci.