RNA-binding protein immunoprecipitation (RIP) assay was performed to prove whether PWRN1 acted as a competitive endogenous RNA (ceRNA) of <i>miR-425-5p</i> Tumor xenograft model and immunohistochemistry (IHC) were developed to study the influence of PWRN1 on tumor growth <i>in vivo</i> Microarray analysis determined that PWRN1 was differently expressed between GC tissues and adjacent tissues. qRT-PCR revealed PWRN1 low expression in GC tissues and cells.
Knockdown of miR-425-5p in GC cells attenuated migration and invasion of GC cells, whereas overexpression of miR-425-5p promoted cell migration and invasion.
Moreover, with interference of miR-425-5p expression in cell lines, we investigated the molecular mechanism of miR-425-5p and the expression level was higher in GC tissues comparing with that in gastric tumor-adjacent mucosa.
Taken together, our results revealed the oncogenic roles of miR-191 and miR-425 in gastric carcinogenesis, and indicated the potential use of serum miR-191 as a novel and stable biomarker for GC diagnosis.