|
Osteosarcoma of bone
|
0.100 |
Biomarker
|
disease |
BEFREE |
In this study, we explored the effects of dioscin on OS in vitro and in vivo and further elucidated the underlying molecular mechanisms and found that dioscin-triggered pyroptosis in GSDME-dependent cell death and that GSDME-N was generated by caspase-3.
|
31535374 |
2020 |
|
Osteosarcoma of bone
|
0.100 |
Biomarker
|
disease |
BEFREE |
The results of the western blot analysis demonstrated that Dox induced increased expression levels of the apoptosis-related proteins cleaved caspase-3 and cytochrome c and loss of mitochondrial membrane potential (MMP) in the U2OS and Saos-2 osteosarcoma cells.
|
24639013 |
2014 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Cell toxicity analysis showed that catechins modification improved the antitumor activity of Se-HAp nanocomposites by inducing apoptosis of human osteosarcoma MNNG/HOS cell lines, through generation of reactive oxygen species (ROS) which in turn activated the caspase-3 pathway, without significantly affecting the growth of human normal bone marrow stem cells (hBMSCs). qPCR and western blot analyses revealed that casp3, p53, and bax genes were significantly upregulated while cox-2 and PTK-2 were slightly downregulated as compared to control in CC/Se-HAp-treated MNNG/HOS cell lines.
|
31263675 |
2019 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The <i>in vitro</i> studies indicated that BDNF-AS overexpression inhibits OS cell proliferation and promotes cell apoptosis through regulating cleaved caspase-3.
|
30352834 |
2018 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The anti-apoptotic property of NS-398 at high dose can be mediated in part through NF-kappaB activation, hsp70 induction and a decrease in caspase-3 activity in human osteosarcoma cells.
|
12012007 |
2002 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Salvianolic acid B suppressed osteosarcoma cell proliferation and induced apoptosis in the osteosarcoma MG63 cell line, and activated the expressions of cleaved caspase-3, phosphorylated-tumor protein (p)38 mitogen-activated protein kinase (p-p38 MAPK) and phosphorylated-p53 (p-p53) proteins in the MG63 cells.
|
29434992 |
2018 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The results suggested that combination of crocin and cisplatin has a strong killing effect on osteosarcoma cells and suppresses the ability of invasion of MG63 and OS732 cells which might be related to up-regulate the expression of caspase-3 and caspase-8.
|
23830991 |
2013 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
This retarded MG-63 osteosarcoma growth in vitro and in vivo in an athymic nude mouse model elicited by Ad-ING4 was closely associated with the increase in the expression of cell cycle-related molecules P21 and P27, decrease in the ratio of anti- to pro-apoptotic molecules Bcl-2/Bax followed by the activation of Caspase-3 leading to apoptosis via intrinsic apoptotic pathways, and the inhibition of tumor angiogenesis.
|
24750000 |
2015 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
This retarded MG-63 osteosarcoma growth in vitro and in vivo in an athymic nude mouse model elicited by Ad-ING4 was closely associated with the increase in the expression of cell cycle-related molecules P21 and P27, decrease in the ratio of anti- to pro-apoptotic molecules Bcl-2/Bax followed by the activation of Caspase-3 leading to apoptosis via intrinsic apoptotic pathways, and the inhibition of tumor angiogenesis.
|
25326586 |
2015 |
|
Osteosarcoma of bone
|
0.100 |
Biomarker
|
disease |
BEFREE |
Furthermore, as compared with parental osteosarcoma 143B cells, the rho(0) cells were found to be more susceptible to apoptosis, which was accompanied by caspase 3 activation and cytochrome c release.
|
15126291 |
2004 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We found that matrine inhibited the proliferation and induced apoptosis of the four osteosarcoma cell lines in vitro and induced the activation of caspase-3, -8, and -9 in a dose-dependent manner.
|
21717192 |
2012 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Proliferation, apoptosis assays and caspase-3 activity assay confirmed that elevated miR-138 expression enhanced osteosarcoma cell chemosensitivity to cisplatin by targeting EZH2.
|
27019355 |
2016 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Meanwhile, CA treatment increased the apoptotic rate and caspase-3/7 activity in osteosarcoma cells in a concentration-dependent manner.
|
30582213 |
2019 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Increases in cell shrinkage, Sub-G1 fragments and the activation of caspase 3 indicated that OGE induced cell apoptosis in U2-OS and HOS cells.
|
24871663 |
2014 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Downregulation of microRNA-95-3p expression suppressed cell growth, induced apoptosis, increased caspase-3 and caspase-9 activities and Bax/Bcl-2 protein expression in osteosarcoma cells.
|
29115549 |
2018 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Using the multiple methods to detect the activity of PF on HOS and Saos‑2 human osteosarcoma cell lines, including an MTS assay, flow cytometry, transmission electron microscopy and western blotting, it was demonstrated that PF induces inhibition of proliferation, G2/M phase cell cycle arrest and apoptosis in the osteosarcoma cell lines in vitro, and activation of cleaved‑caspase‑3 and cleaved‑poly (ADPribose) polymerase in a dose‑dependent manner.
|
29363721 |
2018 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Moreover, WF-A induced a ROS-mediated reduction in mitochondrial membrane potential in a dose-dependent manner and activation of caspase-3 in osteosarcoma cells.
|
28387888 |
2017 |
|
Osteosarcoma of bone
|
0.100 |
Biomarker
|
disease |
BEFREE |
We aimed to investigate whether variants of FasL, MSH2, ABCC5, CASP3 and CYP3A4 are associated with the outcome after chemotherapy-based treatment in osteosarcoma.
|
30065912 |
2018 |
|
Osteosarcoma of bone
|
0.100 |
Biomarker
|
disease |
BEFREE |
After transfection by <i>miR-34</i> mimics, MG-63 cells proliferation in nude mice was significantly impaired (<i>P</i><0.05), and percentage of apoptosis as well as caspase-3 positive cells proportion of osteosarcoma tissue in nude mice was markly increased (<i>P</i><0.05).
|
29895719 |
2018 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Results found that cellular viability was downregulated while caspase 3 activity and expression were promoted in osteosarcoma cells following treatment with various doses of doxorubicin for 24, 48, and 72 h, and the effects showed a dose- and time-dependent manner.
|
26351207 |
2014 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The anticancer effects of HCPT were demonstrated to significantly activate the protein expression of p53, PARP-1 and cytochrome <i>c</i>, and suppress Bcl-2 protein expression and promote the activity of caspase-9 and caspase-3 in human osteosarcoma cells.
|
29434958 |
2018 |
|
Osteosarcoma of bone
|
0.100 |
Biomarker
|
disease |
BEFREE |
Expression of BCL2 apoptosis regulator, BCL2 associated X and caspase‑3 was also involved in chemoresistance development in osteosarcoma.
|
30015899 |
2018 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
WF-A exhibits strong anticancer activity against osteosarcoma cell linesAntiproliferative activity of WF-A is via induction of apoptosisWF-A induced ROS-mediated reduction in mitochondrial membrane potentialWF-A induced expression of caspase-3 in osteosarcoma cells.
|
28839383 |
2019 |
|
Osteosarcoma of bone
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Furthermore, intracellular Bcl-2 protein expression was effectively reduced in 143B-harbored tumor tissue through western blotting analysis (P<0.01), while intratumoral Apaf-1 and cleaved Caspase-3 protein levels were up-regulated, respectively (P<0.01).
|
24797937 |
2014 |
|
Osteosarcoma of bone
|
0.100 |
Biomarker
|
disease |
BEFREE |
Overexpression of miR-377 re-sensitizes cisplatin resistant OS cells to cisplatin-induced caspase-3 dependent apoptosis.
|
29565481 |
2018 |