In contrast, the nuclear localization of P2RY12 was predominant in the higher graded tumors and associated with the expression of the M2 marker CD163.We conclude that P2RY12 is a specific marker for resident microglia in glioma and its expression and localization correspond to tumor grade and predominant stage of M1/M2 immune response.
In this study, via an analysis of public datasets, we demonstrated that CD163 overexpression in glioma specimens correlated with an unfavourable patient prognosis.
Only CD163-positive microglia/macrophages inversely correlate with glioma patients' survival, whereas the total number of microglia does not change with the malignancy grade.
Two SNPs, rs147960238 in CD163 (p = 2.2 × 10<sup>-5</sup>) and rs17138945 in MET (p = 5.6 × 10<sup>-5</sup>) were significantly associated with survival of patients with lower-grade glioma.