Here we show that the E-type prostanoid 4 (EP<sub>4</sub>) receptor expression level is regulated by different concentrations of butyrate, but not by other SCFAs, in human colon cancerHCA-7 cells, through sodium-coupled monocarboxylate transporter-1 (SMCT-1)-mediated uptake followed by the activation of histone acetyltransferase: cAMP response element binding protein-binding protein/p300.
When a targeting module, i.e., the EpCAM aptamer, was incorporated, the 3WJ pRNA nanoparticles were able specifically deliver D5D siRNA to human colon cancerHCA-7 cells both in vitro and in vivo, resulting in significant downregulation of D5D expression.
Four-week old nude mice bearing the human colon cancer cell HCA-7/C29 vs. its delta-5-desaturase knockdown analog (via shRNA transfection) were subject to 4-week treatments of: vehicle control, dihomo-γ-linolenic acid supplementation, 5-Fluorouracil, and combination of dihomo-γ-linolenic acid and 5-Fluorouracil.
In the present study, human colon cancerHCA-7 cells were used as a model system to understand the potential role of EP3 receptors in tumor cell migration.
To elucidate the mechanism of NMF-mediated radiosensitization, we examined the effects of this agent on gamma-ray-induced DNA double-strand breaks and micronuclei in two cell lines, clone A (human colon carcinoma) and HCA-1 (murine hepatocarcinoma).