Abbreviations ADME absorption, distribution, metabolism, and excretion MMGB/SA molecular mechanics generalized born surface area IFD induced fit docking RTK receptor tyrosine kinase NSCLC non-small-cell lung cancer ATP adenosine triphosphate OPLS optimized potential for liquid stimulation RMSD root mean square deviation HTVS high-throughput virtual screening SP standard precision XP extra precision OPLS-AA optimized potential for liquid stimulation-all atom MD molecular simulation MME molecular mechanics energies SGB surface generalized born POPC membrane 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine membrane PDB Protein Data Bank DDR1 discoidin domain receptor 1 DDR2discoidin domain receptor 2 DDRs discoidin domain receptors ECM extracellular matrix TIP4P transferable intermolecular potential 4 point NPT constant particle number, pressure and temperature RMSF root mean square fluctuation Communicated by Ramaswamy H. Sarma.
The mutations in exon 2 of KRAS, exon 18 of DDR2, and exons 5-6 of TP53 genes were screened in lung cancer samples, including non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC) using PCR and sequencing techniques.
In the present study, we have used three molecular methods (ASP-real-time PCR, ASP-DNA-FLA PCR and direct sequencing) to detect the DDR2 gene mutation in 143 patients with NSCLC metastases to the central nervous system (CNS).
Tumour samples from 146 non-small cell lung carcinoma (NSCLC) patients were analysed for relative expression of DDR1 and DDR2 using quantitative real-time PCR (qRT-PCR).