The results showed that patients with high expression of miR-195 had favorable tumor-node-metastasis (late vs early: pooled OR =0.16, 95% CI: 0.11-0.22, <i>P</i><0.001), lymph node metastasis (pooled OR =0.25, 95% CI: 0.18-0.35, <i>P</i><0.001) and distant metastasis (pooled OR =0.26, 95% CI: 0.13-0.52, <i>P</i><0.001).
Of 4 miRNA targets tested (miR-181a, miR-329, miR-331, miR-195), mir-331 was significantly over-expressed in patients with metastatic disease, compared to patients with local disease (p < 0.001) or healthy controls (p < 0.001). miR-195 was significantly under-expressed in patients with metastatic disease, compared to patients with local disease (p < 0.001) or healthy controls (p = 0.043).
These findings indicated that expression of miRNA-195 in laryngeal carcinoma tissue is down-regulated, and the low expression of miRNA-195 may be related to invasion and metastasis of laryngeal carcinoma, which indicates poor prognosis of patients.
We found that miR-195 directly targets <i>TUBB</i>; knockdown of <i>TUBB</i> sensitizes cells to MTAs, while overexpression confers resistance; high expression of <i>TUBB</i> is correlated with worse survival of lung adenocarcinoma; TUBB is also regulated by CHEK1, which has been shown to regulate chemoresistance; and miR-195 targets <i>BIRC5</i> to repress migration and invasion <i>in vitro</i> and metastasis <i>in vivo</i>.
The tumor suppressive ability of miR‑195 in rectal cancer cell proliferation and metastasis was mediated by blocking IGF1 expression and inhibiting the PI3K/AKT pathway.
In the present study, miR‑195 was significantly downregulated in cervical cancer tissue samples compared with adjacent non‑tumor tissue samples, and the reduced expression level of miR‑195 was associated with node metastasis and an advanced clinical stage in cervical cancer.
Lastly, we revealed YAP was not only the downstream of miR-195 in HCC, but also mediated the promoting effects of miR-195 on the metastasis and EMT of HCC cells.
miR-195 downregulation in prostate cancer tissues was significantly associated with high Gleason score (P = 0.001), positive metastasis failure (P < 0.001), and biochemical recurrence (BCR, P < 0.001).
MicroRNA-195 (miR-195) plays important roles in tumor metastasis and angiogenesis, yet its function and mechanism of action in hepatocellular carcinoma (HCC) remain to be elucidated.
In summary, our study suggests miR-195 functions as a tumor metastasis suppressor gene by down-regulating CCND1 and can be used as a potential target in the treatment of osteosarcoma.
In conclusion, aberrant expression of clustered miRNAs was identified by deep sequencing, and downregulation of miR-195/497 contributed to BC progression and metastasis.