|
Central neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Our findings provide evidence for distinct patterns of Op18 phosphorylation in neuroblastoma tumors with and without N-myc gene amplification.
|
2267130 |
1990 |
|
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
These results demonstrate that enhanced MYCN expression contributes to malignant progression of human neuroblastoma cells, conceivably by stimulating the expression of autocrine growth factor activity.
|
2364393 |
1990 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We assessed tumor cell DNA content (ploidy) and N-myc gene copy number as predictors of long-term disease-free survival in 298 children with neuroblastoma.
|
2066755 |
1991 |
|
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
These results suggest that the down-modulation of the MHC class I expression may be associated with the high level of expression and amplification of N-myc gene in the advanced stage of neuroblastoma.
|
1991301 |
1991 |
|
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We report that untreated neuroblastoma tumors contain a cellular polypeptide (Mr = 19,000) designated p19, identified in two-dimensional electrophoretic gels, which occurs at significantly higher levels (P = 0.0001) in primary tumors containing amplified N-myc gene.
|
2056128 |
1991 |
|
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Our results demonstrate that enhanced MYCN expression confers to neuroblastoma cells the ability to respond to bFGF, possibly by inducing functional FGF receptors.
|
1656953 |
1991 |
|
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We conclude that the immunohistochemical detection of N-myc protein expression is one of the most unfavorable prognostic factors in neuroblastoma patients.
|
1895195 |
1991 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Transfected clones were isolated and found to express the N-myc gene at levels similar to those seen in a tumor cell line (neuroblastoma LA-N-1) which contains an amplified N-myc gene.
|
1861869 |
1991 |
|
Central neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Neuroblastoma with DNA amplification and rearrangement in the N-myc gene region.
|
2004381 |
1991 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
The MYCN protein of human neuroblastoma cells is phosphorylated by casein kinase II in the central region and at serine 367.
|
1923500 |
1991 |
|
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Expression of protein kinase C-alpha (PKC-alpha) and MYCN mRNAs in human neuroblastoma cells and modulation during morphological differentiation induced by retinoic acid.
|
2013316 |
1991 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
MYCN amplification does not affect survival of neuroblastoma patients treated with autologous bone marrow transplantation.
|
1855077 |
1991 |
|
Central neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
To study the regulation of N-myc, we have subcloned fragments of the 5' flanking region of the human N-myc gene upstream of the chloramphenicol acetyl transferase (CAT) reporter gene, and assayed for promoter activity in transient transfections into neuroblastoma and other cell lines.
|
1565467 |
1992 |
|
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
In this report, the upstream region of mouse N-myc gene was ligated to pSVPCAT, which carries the simian virus 40 (SV40) promoter and bacterial chloramphenicol acetyltransferase (CAT) gene, and transcriptional activities were examined by CAT and S1 protection assays after transfection of the DNAs into human cervical carcinoma HeLa or neuroblastoma IMR32 cells.
|
1327147 |
1992 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We have used the polymerase chain reaction (PCR) to detect amplification of the MYCN oncogene in neuroblastoma cell lines and to distinguish primary tumors with a single copy from those with MYCN amplification using DNA extracted from frozen sections.
|
1342970 |
1992 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Applying this technique, we followed the metaphase location and interphase position of amplified DNA sequences corresponding to the SAMK, MYC, and MYCN genes in four cell lines derived from human tumors: two gastric carcinoma lines (KATO III and SNU-16), a neuroblastoma (NUB-7), and a neuroepithelioma (NUB-20) line.
|
1377938 |
1992 |
|
Central neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
One, favorable neuroblastoma, is associated with young age and early stage at diagnosis, triploid karyotypes, no 1p abnormalities or N-myc gene amplification, more mature catecholamine synthesis and excretion, and excellent clinical outcome despite no or minimal therapy.
|
1727993 |
1992 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Detection of MYCN amplification in three neuroblastoma cell lines by non-radioactive chromosomal in situ hybridization.
|
1581879 |
1992 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In our investigation of 14 neuroblastoma patients, we could demonstrate N-myc gene amplification in the bone marrow of 5 patients with neuroblastoma stage IV disease and in bone marrow infiltration without enrichment of neuroblastoma cells.
|
1458508 |
1992 |
|
Central neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Evaluations of N-myc gene amplification and DNA ploidy are complementary, and the combined determination of these two factors may be one of the most powerful prognostic indicators in neuroblastoma.
|
8402501 |
1993 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Detection of N-myc gene amplification by fluorescence in situ hybridization. Diagnostic utility for neuroblastoma.
|
7684192 |
1993 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Structural organization of MYCN amplicons of neuroblastoma tumors, xenografts, and cell lines characterized by the sequences encompassing the MYCN amplicons in a human neuroblastoma cell line.
|
7691154 |
1993 |
|
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
N-myc gene amplification/expression in localized stroma-rich neuroblastoma (ganglioneuroblastoma).
|
8454274 |
1993 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Determination of N-myc gene amplification in neuroblastoma by differential polymerase chain reaction.
|
8366868 |
1993 |
|
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
A method is described to estimate MYCN (N-myc) oncogene amplification in neuroblastoma by the technique of differential polymerase chain reaction (PCR).
|
8443050 |
1993 |