|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
These observations suggest there may be divergent mechanisms for Tpm binding to actin filaments, where conformational flexibility in mammalian Tpm allows a preorganized shape complementary to the filament surface, and where rigidity in the crustacean Tpm requires concerted bending and binding.
|
31390486 |
2020 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
From the deformation profiles, we determined key biophysical properties of tethered actin bundles such as their rigidity and filament density.
|
31791547 |
2020 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
Tks5 and Dynamin-2 enhance actin bundle rigidity in invadosomes to promote myoblast fusion.
|
30894403 |
2019 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
Regardless of the viscoelastic model applied, increasing substrate rigidity was related to an overall increase in cell stiffness and apparent viscosity (2.95 ± 1.56 kPa and 921.45 ± 102.46 Pa.s for the stiff substrate; 2.17 ± 1.30 kPa and 557.37 ± 494.11 Pa.s for the intermediate substrate), associated with an organized actin cytoskeleton.
|
30242964 |
2019 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
We will describe our strategy for the structural analyses of actin filament and actomyosin rigor complex and the biological insights we gained from these structures.
|
30617822 |
2019 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
Moreover, cortical branched actin more generally determines S-phase entry by integrating soluble stimuli such as growth factors and mechanotransduction signals, ensuing from substratum rigidity or stretching of epithelial monolayers.
|
30971746 |
2019 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
It is compatible with the actin-attached myosin heads being in a different conformation on actin, with the average centre of cross-bridge mass at a higher radius than in normal rigor and the myosin lever arms conforming less to the actin filament geometry, probably pointing back to their origins on their parent myosin filaments.
|
30022010 |
2018 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
Addition of blebbistatin to MDCK cells to reduce cortical actin rigidity resulted in a decrease in cell elongation across all curvatures while preserving the biphasic trend.
|
29642039 |
2018 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
Actin and nesprin are essential for maintaining deformed nuclei, while lamin A/C and intermediate filaments confer rigidity to the nucleus.
|
29756146 |
2018 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
Cell response to matrix rigidity has been explained by the mechanical properties of the actin-talin-integrin-fibronectin clutch.
|
29358406 |
2018 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
We also observed that the rate of binding of rigor subfragment 1 to pyrene-labeled regulated actin at saturating Ca<sup>2+</sup> was higher for the truncation mutants than for wild-type TnT.
|
30057009 |
2018 |
|
Muscle Rigidity
|
0.100 |
GeneticVariation
|
phenotype |
BEFREE |
Our computational study showed that D286G impairs the flexural rigidity of actin filaments.
|
30564146 |
2018 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
We find that nuclear positional dynamics is sensitive to the cytoskeletal organization by studying the effect of actin polymerization and nuclear rigidity on the diffusive behavior of the nucleus.
|
28494962 |
2017 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
These results indicate that the same signaling molecules activated by E-cadherin rigidity sensing on PA gels contribute to actin organization and membrane dynamics during cell-cell adhesion.
|
28674019 |
2017 |
|
Muscle Rigidity
|
0.100 |
GeneticVariation
|
phenotype |
BEFREE |
Superimposition of myo2-E1 and myo2-E1-Sup1 on available rigor and blebbistatin-bound myosin II structures suggests that myo2-E1-Sup1 may represent a novel actin translocation-defective allele.
|
28238661 |
2017 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
Here, we report the 8 Å resolution structure of the rigor (nucleotide-free) actin-tropomyosin-myosin complex determined by cryo-electron microscopy.
|
22817895 |
2012 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
Thus, F-actin bundling by TRIOBP provides durability and rigidity for normal mechanosensitivity of stereocilia and may contribute to resilient cytoskeletal structures elsewhere.
|
20510926 |
2010 |
|
Muscle Rigidity
|
0.100 |
Biomarker
|
phenotype |
BEFREE |
The histograms of fluctuations of fluorescent actin molecules in wild-type (WT) hearts in rigor were represented by perfect Gaussian curves.
|
20509708 |
2010 |