As a result, the miRNA-TF-mRNA regulatory network, including 1049 nodes (516 miRNA, 25 TFs, and 508 DEGs) and 15942 edges (interaction relationships, such as Pparg-Abca1 and miR-590-3p-AXIN2), was constructed, from which three significant modules were extracted and modules 2 and 3 contained interactions between miRNAs/TFs and DEGs such as miR-103-3p-<i>AXIN2</i>, miR-124-3p-<i>AR</i>-<i>Tgfb1i1</i>, and miR-27a-3p-<i>PPARG</i>-<i>Abca1</i>. miR-27a-3p was a known miRNA associated with OS.
In this study, we show that miR-590-3p was significantly decreased both in osteosarcoma tissues and cell lines, suggesting a potential role of miR-590-3p in osteosarcoma.