Hepatitis C
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
We also characterized wild-type and Jc1/H77 hypervariable region 1 (HVR1)-swapped mutant HCVcc particles produced in serum-free media and incubated with different serum types or with purified lipoproteins.
|
30553840 |
2019 |
Hepatitis C
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
We cultured HCV core-NS2 recombinants H77 (genotype 1a)/JFH1 or the highly antibody-susceptible hypervariable region 1 (HVR1)-deleted variants H77/JFH1∆HVR1 and J6(genotype 2a)/JFH1∆HVR1 in Huh7.5 cells with AR4A.
|
30102355 |
2019 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
In conclusion, our study indicates that HVR1 and glycans regulate HCV neutralization by shifting the equilibrium between open and closed envelope conformations.
|
31040211 |
2019 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Thus, we identify novel AR3A-specific resistance substitutions and the role of HVR1 in protecting HCV from AR3-targeting antibodies.
|
30487284 |
2019 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
The aim of the present study was to determine the genetic variability of HCV HVR1 (hypervariable region 1) of genotype 1b and 3 in plasma of blood donors in the early seronegative stage of infection (HCV-RNA+, anti-HCV-) and in samples from chronically infected patients using next-generation sequencing.
|
31492939 |
2019 |
Hepatitis C
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
We created hepatitis C virus variants with mutated glycosylation sites and/or hypervariable region 1 (HVR1).
|
30439392 |
2019 |
Hepatitis C
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Nucleotide (nt) sequences of intra-host HCV HVR1 variants (N = 28,622) obtained from CIP (N = 112) and MIP (n = 176) were represented using 148 physical-chemical (PhyChem) indexes of DNA nt dimers.
|
30075255 |
2018 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
NH DPHS conducted site visits, case patient and employee interviews, medical record and medication use review, and employee and patient HCV testing using enzyme immunoassay for anti-HCV, reverse-transcription polymerase chain reaction for HCV RNA, nonstructural 5B (NS5B) and hypervariable region 1 (HVR1) sequencing, and quasispecies analysis.
|
29767683 |
2018 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Our results indicate that the ΔHVR1 gpE1/gpE2 antigen confers no advantages in the neutralization of HCV compared with the WT antigen.
|
29540595 |
2018 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
HCV hypervariable region 1 (HVR1) was amplified in seven patients (including one anti-HCV positive) and analyzed by next generation sequencing (NGS).
|
29566084 |
2018 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
To fully elucidate the multifunctional role of HVR1 in HCV entry and NAb evasion, improved E1/E2 models and comparative studies with other NAb evasion strategies are needed.
|
30319614 |
2018 |
Hepatitis C
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
The occurrence of five sense mutations [S391A, G397A, L402F and M405T in the hypervariable region 1 (HVR1) of envelope glycoprotein 2 and I2750M in NS5B] suggested that HCV undergoes genetic evolution during culture.
|
28758632 |
2017 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Here, we describe a new sequence alignment-free method to discriminate between recent (R) and chronic (C) HCV infection using next-generation sequencing (NGS) data derived from the HCV hypervariable region 1 (HVR1).
|
29244000 |
2017 |
Hepatitis C
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Point mutations of the HVR1 can lead to humoral immune escape in HCV-infected patients.
|
28284234 |
2017 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Thus, we were able to take advantage of the neutralization-sensitive HVR1-deleted viruses to rapidly generate escape viruses aiding our understanding of the divergent escape pathways used by HCV to evade AR5A.
|
28231271 |
2017 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Sequencing of multiple and longer sub-genomic regions has been proposed as an alternative to overcome the limitations imposed by the rapid molecular evolution of the HCV HVR1.
|
26733442 |
2016 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
We obtained sequences from the HCV hypervariable region 1 (HVR1), using end-point limiting-dilution (EPLD) technique, from 127 cases involved in 32 epidemiologically defined HCV outbreaks and 193 individuals with unrelated HCV strains.
|
26582955 |
2016 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
The HRM analysis of the amplified cDNAs encoding the PKR-BD and HVR1 allowed the detection of partial replacement of HCV-1b by HCV-1a subspecies in one of our patients, as well as evaluation of the effectiveness of pegylated interferon α/ribavirin (PEG-IFNα/RBV) therapy.
|
25380691 |
2015 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
We have studied the immune reactivity of hypervariable region 1 (HVR1) of hepatitis C virus (HCV) against HCV immune positive and negative sera.
|
26330263 |
2015 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Analysis of HVR1 sequences from intra-host HCV variants obtained by NGS showed that three donors were infected with >1 HCV strain, including infections with 2 genotypes.
|
26683463 |
2015 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Sanger sequencing (HCV NS5B, HVR1 and Core-E1-HVR1) and phylogenetics were applied to samples from individuals diagnosed with HCV in British Columbia, Canada in 2011.
|
25917496 |
2015 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Hypervariable region 1 (HVR1) of hepatitis C virus (HCV) comprises the first 27 N-terminal amino acid residues of E2.
|
26719263 |
2015 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Thus, this exploratory study evaluated diversity of the hypervariable region 1 (HVR1) of HCV in the plasma and liver for 14 patients co-infected with HIV and HCV.
|
24788693 |
2014 |
Hepatitis C
|
0.100 |
Biomarker
|
disease |
BEFREE |
Examination of intra-host viral populations using next-generation sequencing of the HCV HVR1 showed a significant variation in intra-host genetic diversity among infected individuals, with some strains composed of sub-populations as distant from each other as viral populations from different hosts.
|
24519518 |
2014 |
Hepatitis C
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
The HCV quasispecies from the control group had a higher frequency of variable sites in HVR1 (83.9 % vs 59.3 %, p < 0.05), as well as a greater diversity within (intra-patient) and between samples, compared to the CRF group.
|
24562426 |
2014 |