As a result, the miRNA-TF-mRNA regulatory network, including 1049 nodes (516 miRNA, 25 TFs, and 508 DEGs) and 15942 edges (interaction relationships, such as Pparg-Abca1 and miR-590-3p-AXIN2), was constructed, from which three significant modules were extracted and modules 2 and 3 contained interactions between miRNAs/TFs and DEGs such as miR-103-3p-<i>AXIN2</i>, miR-124-3p-<i>AR</i>-<i>Tgfb1i1</i>, and miR-27a-3p-<i>PPARG</i>-<i>Abca1</i>. miR-27a-3p was a known miRNA associated with OS.
Moreover, Axin2 was identified to be a functional downstream target of miR-374a, and decreased expression of Axin2 could promote OS cell proliferation.