|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
Here we examined the regulation of HIF-1alpha by hypoxia and nitric oxide (NO) in explants of human cerebrovascular smooth muscle cells.
|
14657004 |
2004 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
Proteasomal activity in placentas from women with preeclampsia and intrauterine growth restriction: implications for expression of HIF-alpha proteins.
|
18222538 |
2008 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
These data suggest that hypoxia enhances the expression of MUC1 through the transcriptional regulation by HIF-1alpha in a human lung epithelial cell line.
|
19141292 |
2009 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Chromatin immunoprecipitation assay demonstrated that HIF-1 alpha directly bound to this region under normoxia, and this binding activity was significantly enhanced under hypoxia.
|
18339685 |
2008 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
This trans-regulation between HIF-1alpha and HIF-2alpha during hypoxia likely conveys target gene specificity.
|
14744852 |
2004 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
In response to hypoxia, HIF-1 bound to this site and transactivated SAG expression.
|
17828303 |
2008 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Transfection with specific siRNA duplexes knocked down HIF 1-alpha mRNA and protein expression in hypoxia-exposed cells by approximately 80%, whereas transfection with scrambled siRNA duplexes had no noticeable effect on HIF 1-alpha expression.
|
16779673 |
2006 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Treatment of cells under hypoxia with low concentrations of the superoxide generator 2,3-dimethoxy-1,4-naphthoquinone lowered HIF-1alpha protein stabilization.
|
16140212 |
2005 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
In conclusion, our data demonstrate that hyperglycemia impairs hypoxia-dependent protection of HIF-1alpha against proteasomal degradation and suggest a mechanism by which diabetes interferes with cellular responses to hypoxia.
|
15561954 |
2004 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
These results suggest that the hypoxia induces the migration of CASMCs, and that the migration is elicited by TSP-1 of which induction is fully dependent on the stabilization of HIF-1alpha, in autocrine regulation.
|
18384112 |
2008 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Chromatin immunoprecipitation identified a previously unappreciated binding site for the hypoxia inducible factor-1 (HIF-1), and promoter studies established its relevance by loss of repression following point mutation.
|
18779379 |
2009 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
Impact of HIF-1alpha and HIF-2alpha on proliferation and migration of human pulmonary artery fibroblasts in hypoxia.
|
16263938 |
2006 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Dimethyloxallyl glycine, a stabilizer of hypoxia-inducible factor 1alpha (HIF1alpha), mimicked the hypoxia-mediated upregulation of visfatin, and YC1, an inhibitor of HIF1 cancelled the hypoxia-induced upregulation of visfatin mRNA.
|
16970912 |
2006 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
HUVECs were transfected with specific 21-nt small interfering RNA (siRNA) duplexes targeting HIF-1 alpha mRNA sequences or scrambled RNA duplexes and subjected either to normoxia for 251/2 h or to anoxia for 11/2 h, and subsequently normoxia for 24 h (A/R).
|
15064771 |
2004 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
We suggest a dual role for HIF-1alpha in providing a survival or death signal, based on hypoxia duration, and consider the nuclear transcription factor, CREB, to be a possible target for hypoxic therapy against leukemia disease.
|
18996491 |
2009 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Using the ChIP assay, we discovered that BRCA1 could be recruited to the endogenous human VEGF promoter along with HIF-1alpha following hypoxia.
|
16543242 |
2006 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
In contrast, coexpression of HIF-1alpha and CAIX in the epithelium in phyllodes tumors points to epithelial hypoxia, most probably caused by relatively distant blood vessels.
|
16168127 |
2005 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Overexpression of HIF-1alpha and HIF-2alpha was demonstrated in three HNSCC cell lines under hypoxia and tumor tissue versus normal tissue (n = 20, HIF-1alpha, P = 0.023; HIF-2alpha, P = 0.013).
|
11980639 |
2002 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
In this study, we have investigated the potential roles of RORalpha in the hypoxia signaling pathway in connection with activation of HIF-1alpha.
|
18658046 |
2008 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Taken together, these results demonstrate transcriptional coordination of A2BR by HIF-1alpha and amplified adenosine signaling during hypoxia.
|
17077301 |
2006 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
Hypoxia stimulates hypoxia-inducible factor-1alpha (HIF-1alpha), a transcriptional activator of VEGF.
|
12401798 |
2002 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
The results showed that, although hypoxia markedly increased ROS generation in HeEB1 cells but not in EB8 cells, EB8 cells showed essentially a normal response to hypoxia, as assessed by VEGF gene promoter activity, HIF-1alpha accumulation, and HIF-1 target gene expressions.
|
12237125 |
2002 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Cell density also correlated with increased staining for reductively activated pimonidazole (a marker for hypoxia), as well as with increased levels of the HIF-1alpha subunit and HIF transcriptional activity as determined by immunocytochemistry, Western blot, and luciferase reporter assays.
|
17289019 |
2007 |
|
Anoxia
|
0.100 |
AlteredExpression
|
phenotype |
LHGDN |
Herein, we identify a mechanism of increased LT in SCD and show HIF-1alpha as a hypoxia-independent target of PlGF.
|
18945963 |
2009 |
|
Anoxia
|
0.100 |
Biomarker
|
phenotype |
LHGDN |
The transcription-dependent degradation of HIF-1alpha under hypoxia occurs via the proteasome and is accelerated by protein acetylation.
|
15897903 |
2005 |